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[5] These investigations will help the professional determine the severity of insomnia symptoms and what factors might be contributing to these symptoms

Coronal slices (300 m thick) containing the CeA were prepared with a Leica VT1000S (Leica Microsystems) and incubated in oxygenated (95% O 2 /5% CO 2 ) artificial CSF (aCSF) containing the following: 120 mm NaCl, 2.5 mm KCL, 5 mm EGTA, 2.0 mm CaCl 2 , 1.0 mm MgCl 2 , 1.2 mm NaH 2 PO 4 , 26 mm NaHCO 3 , 1.75 mm glucose, and 5 mm HEPES for 30 min at 37C, followed by 30-min equilibration at room temperature (RT

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