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They added 333 commonly used additives to the 1R4F reference cigarette and measured effects of ingredients

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The comparisons between the sensitivity thresholds of the various groups were analyzed using the Kruskal-Wallis test (KW) and a linear mixed model covering each locus for a global comparison of repeated measures (The assumption of normality of residuals was not met, nevertheless a mixed model is still robust in terms of parameter estimation) and the MannWhitney U-test (MW) for individual loci comparisons

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