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Cigarette smoke extract (CSE) preparation CSE was prepared by burning one 3R4F reference cigarette, bubbling the direct and side-stream smoke into a 50 ml conical tube containing 25 ml of DMEM/F12 (Gibco, Cat#11320-033) without FBS and antibiotics using a peristaltic pump (Precision Blood Pump, COBE Perfusion System) at 0.12 rpm

This on the other hand has a little extra

the cigar was a sign of authority, wealth and power

Of the 61 countries considered in the report, 59 have ratified an international treaty called the WHO's Framework Convention on Tobacco Control, which recommends evidence-based measures for reducing numbers of young people who smoke