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This estimate is higher than the prevalence of flavored cigarette use reported among smokers in studies from the United States 28 and the European Union 26

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Human LIF and mouse Lif gene expressions were performed by RT-PCR and corrected to ACTB or Actb using the following primers: human LIF forward 5-GAA GAA GCT GG CTG TCA A-3, human LIF reverse 5-ACA TCT GGA CCC AAC TCC T-3, human ACTB forward 5-GAT GAG ATT GGC ATG GCT-3, human ACTB reverse 5-CAC CTT CAC CGT TCC AGT-3, mouse Lif forward 5-TAG GAG TCA GGG AAG GAC-3, mouse Lif reverse 5-GAC AGC TGT GCT GGA TCA-3, mouse Actb forward 5-GTT GGA GCA AAC ATC CCC CA-3, mouse Actb reverse 5-CGC GAC CAT CCT CCT CTT AG-3 (Life technologies/Applied Biosystems, Carlsbad, CA)

11 Youth (ages 12-18) While behavioral interventions are the first line treatment option in this population, a risk benefit assessment may indicate a need for pharmacotherapy, of which NRT is the recommended option