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Appl Microbiol Biotechnol 36:538840 Awano N, Wada M, Kohdoh A, Oikawa T, Takagi H, Nakamori S (2003) Effect of cysteine desulfhydrase gene disruption on l-cysteine overproduction in Escherichia coli

People always say, "Oh, if you apply on the thinner part of skin, does it absorb better or not?" No, this is a water-soluble product

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One approach is to position the GST tag at the N-terminus or C-terminus of the target protein, depending on which end is less likely to affect the protein's function