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Moreover, the results of in vivo experiments showed that nude mice treated with HGC-27R+DIDS-EVs/PTX exhibited significantly decreased tumor volume and weight compared to mice receiving HGC-27R-EVs/PTX ( Figures 6F, G and S3G )

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This saturation threshold suggests a ceiling dose exists, beyond which additional Dihexa provides no incremental benefit but does increase systemic exposure and potential off-target binding