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The erythrocytes were then lysed with lysis buffer (BD Pharm Lyse, BD Bioscience, San Jose, USA) for 10 min followed by centrifugation (250 x g, 5 min, 4C, Centrifuge 5427 R, Eppendorf SE, Hamburg, Germany) and resuspension of the cells in Hepes-buffered saline (HBS, 14 mM Hepes, 0.9% NaCl)
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For protein characterization studies primers containing BamHI and XhoI restriction sites were used to amplify both 57 Cr GPDH 705 and 87 Cr GPDH 705 constructs from a pUC57 vector which contained H
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