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The agonists against ionotropic glutamate receptors 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 M) and aminophosphonopentanoic acid (AP-5, 50 M) were used to isolate inhibitory postsynaptic currents (IPSC)

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Methods ICU patient blood collection The prospective cohort study in adult critically ill patients was approved by the Ethical Committee of the Ghent University Hospital (Belgian registration number of the study: B670201213147), and conducted in accordance with the declaration of Helsinki and in compliance with the Good Clinical Practice Guidelines