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The SBP-tag enabled binding to NeutrAvidin beads, which facilitated treatment with Na 2 S 2 , followed by washing and processing without precipitation and resuspension of the protein (Supplementary Fig

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Of note is that the results of this trial are somewhat conflicting, and the study design does not allow the effects of riboflavin and niacin to be differentiated

In order to provide further evidence that the oxidized species of strep-MBP-ROXY9 indeed contains the expected disulfide between Cys21 and Cys24, strep-MBP-ROXY9 variants with either SCLC or CCLS sequences in the active site were analysed (Supplementary Fig