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Once inside, the unprotonated amphetamine molecules become rapidly protonated by the acidic environment of the vesicle, which alkalinizes the vesicle matrix and completely dissipates the necessary pH gradient (Wimalasena 2011)(see Figure 2 Mechanism A)

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Additionally, histological assessment involved Tyrosine Hydroxylase (TH) staining in the substantia nigra to evaluate the integrity and potential restoration of dopaminergic neurons, with evaluations performed at 1 month and 3 months post-lesion

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