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At the cellular level, the juxtaposition of these two peptides allows analyses to be conducted on different but related mechanisms

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First, in vitro studies predominantly use immortalized cell lines (e.g., HepG2, L02) or primary hepatocytes under simplified lipid overload conditions, failing to recapitulate the complex in vivo microenvironment of NAFLDincluding crosstalk between hepatocytes, hepatic stellate cells, and immune cells, or systemic metabolic dysregulation

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