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In step-b), Fmoc-Ser(tBu)-Tyr(tBu)-Leu-Glu(OtBu)-Gly-OH (fragment- 3) was condensed with peptide obtained from step-a) in presence of a coupling agent to obtain Fmoc-Ser(tBu)-Tyr(tBu)- Leu-Glu(OtBu)-Gly-Gln(Trt)-Ala-Ala-Lys(C18 diacid mono-t-butyl-y-Glu(AEEA-AEEA)- OtBu)-Glu(OtBu)-Phe-Ile-Ala-Trp(Boc)-Leu-Val-Arg(pbf)-Gly-Arg (pbf)-Gly-OtBu

( H ) Influence of SQR depletion on the loss of viability of HeLa cells treated with either RSL3 (5 M) (left panels) or CHP (40 M) (right panels), measured with the CellTox Green cytotoxicity assay

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