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Metformin treatment significantly reduced the percentage of live HCT116 and SW837 cells following irradiation with 1.8 Gy, when compared to irradiated vehicle controls ( Figure 3A )

By utilizing the home-made reversed-phase column, we then conducted Liquid Chromatograph Mass Spectrometry (LCMS/MS) analysis was later conducted with a gradient of solvents A (2% acetonitrile, 0.1% formic acid within water) and B (90% acetonitrile, 0.1% formic acid within water) using the EASY-nLC 1200 UPLC system

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