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Particles can come from: Degraded peptide forming insoluble aggregates Contamination during manufacturing or storage Rubber stopper fragments from repeated needle punctures Crystallization of dissolved components If you see particles, do not try to filter them out

It is also reported that non-cell-permeable cryoprotectants can exert their efficacy by stabilising the plasma membranes 23,24

Furthermore, despite several studies providing interesting data, this association should be interpreted with caution because the majority of these studies did not report or adjusted for the effect of ITB in their survival analyses [24]