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Longer cycles should include careful self-monitoring and ideally involve periodic blood work including copper levels

For the calibration standard and quality control samples, 5 l aliquot of each CS and QC solutions was combined with 95 l of blank plasma, vortexed for 30 s before 50 l of IS working solution (dexketoprofen, 1 g/ml) and 350 l of methanol were added to the plasma samples, which were mixed via vortex for 1 min and centrifuged at 20,200 g for 10 min at 4C

Perform clinical examination and biopsy of suspicious unexplained lesions

Studies show that patients typically regain 50-70% of lost weight within one year of stopping treatment