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doi: 10.1016/s0140-6736(96)91273-8

Cao, Z., Cheng, S., Wang, X., Pang, Y

GIAD 2:225239 Jungmichel S, Rosenthal F, Altmeyer M, Lukas J, Hottiger MO, Nielsen ML (2013) Proteome-wide identification of Poly(ADP-ribosyl)ation targets in different genotoxic stress responses

In vitro PARylation assays For the in vitro PARylation assay, purified GST-RSMC or GST-RSMC-16A was bound to Glutathione Sepharose 4B beads and incubated with the PARP1 enzyme (Sino Biological, 11040-H08B) for 1 h at 37 C in reaction buffer (50 mM TrisHCl pH 8.0, 150 mM NaCl, 10 mM MgCl 2 , 10% glycerol, 1 mM DTT) supplemented with or without 200 M NAD + , followed with three times wash with reaction buffer supplied with 300 mM NaCl