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Previous studies of human omega class GSTs and OaGST showed that there are two GSH binding sites, referred to as the G-site (GSH binding) and the H-site (hydrophobic binding) 18,20,21 (Supplemental Fig

The oligomer was then diluted 1/24 with a solution of TRIS 10 mM pH 7.5 to afford a final concentration of 10 M and incubated at 37 C (8.3 L of the oligomer stock solution was diluted with 190 L of TRIS 10 mM pH 7.5 and 1.7 L of the pancreatin solution)

It's the first checkpoint for data reliability

Whether this pattern persists beyond 52 weeks, whether it is dose-dependent, and whether it is reversible upon discontinuation of therapy are questions that remain unanswered and need to be addressed in larger, longer-duration trials