A guide for measurement of circulating metabolic hormones in rodents: Pitfalls during the pre-analytical phase
The most pronounced differences were observed in gastrointestinal and metabolic events: nausea (combination: n = 9651 vs monotherapy: n = 38,030), vomiting (4383 vs 17,062), pancreatitis (1389 vs 6655), and hypoglycemia (773 vs 1778)
Samples obtained from the same time point and same treatment group were pooled in BD Microtainer SST Tubes (#365968, Becton, Dickson and Company, Franklin Lakes, New Jersey, USA), spun down, and the serum stored at 80 C for colistin composite pharmacokinetics
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