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The biotinylated complexes were dialyzed against NaCl 150 mM, Tris 10 mM pH 8.0, concentrated and tested by HPLC on a gel filtration column (Cytiva) for determining monomer purity and percentage of free 2m

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Collectively, our observations indicate a significant involvement of cryptic glycan epitopes in immunological recognition of senescent beta cells, and that clonal composition of the GlcNAc-specific B cell repertoire during early life can significantly influence immune responsiveness to Type 1 diabetes autoantigens, Kearney said

The concentration of Rat IFNg in the samples is then determined by comparing the OD of the samples to the standard curve