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The membranes were blocked for 1 h at room temperature in 5% non-fat dry milk (4259.01, SERVA, Heidelberg, Germany) prepared in Tris-buffered saline with Tween-20 (P7949, Sigma-Aldrich, Pozna, Poland) (TBST)

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The clinical programme ended in 2007

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As a result, vitamin B12 deficiency is relatively common, especially among older people

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