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Also, not having a risk factor does not mean that an individual will not get the condition

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The membranes were blocked using Bullet Blocking One for Western Blotting (Nacalai Tesque), incubated overnight at 4C with the primary antibody, and then incubated with an HRP-conjugated secondary antibody

The temperature-dependent increase in the rate of hydrolysis of pNPB and pNPPal is likely to be extremely complex, involving contributions from the increase in k T (Arrhenius behaviour), as well as from temperature-induced reduction in viscosity, leading to increased substrate and product diffusion rates