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To monitor luciferase reactivation, 1 L of the refolding mixture was added to 60 L of Luciferase Assay Reagent (Promega, USA), and luminescence was measured using a Cytation 5 Multi-Mode Reader (Agilent BioTek)

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Tirzepatides cardiovascular benefits are still being studied, but early data suggests it offers similar protections

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