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Based on preclinical (animal and cellular) data only, inhibition of NNMT by 5-Amino-1MQ is theorised to: Raise intracellular NAD+ levels Activate SIRT1 and other sirtuins (proteins linked to metabolic regulation) Reduce fat cell (adipocyte) size and proliferation Improve insulin sensitivity These effects have been demonstrated in mouse models (Neelakantan et al., 2019), but robust human clinical trial data are currently absent

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Specifically, he outlined four potential mechanisms through which AOD-9604 could be applied to osteoarthritis and cartilage/bone repair: Stimulates bone differentiation and mineralization in adipose-derived mesenchymal stem cells (MSC) Promotes myoblast differentiation Promotes chondrocyte production of collagen and proteoglycan Stimulates stem cell differentiation toward bone, muscle, and cartilage repair One peptide physician on Reddit shared his results with doing so and reported positive results: This is exactly what I use in practice