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Lencer (Boston Childrens Hospital, Harvard Medical School and Harvard Digestive Diseases center, United States) were cultured in MCDB 131 medium (Gibco) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Gibco), 2 mM l-glutamine (Gibco), 25 g/mL streptomycin (Gibco), 25 U/mL penicillin (Gibco), 10 ng/mL recombinant mouse epidermal growth factor (Gibco), and 1 g/mL hydrocortisone (Sigma-Aldrich), at 37 C in a humidified atmosphere of 5% CO 2

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The study further examined the impact of Goralatide on the proliferative activity of normal, remission, and leukemic progenitor cells, illustrating that under control conditions, erythroid progenitor cells are highly proliferative, with 38% of CFU-E and 30% of BFU-E cells in the S-phase of the cell cycle

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