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The conditions used were as follows: Product ion scan in positive mode, precursor m/z 271 for APAP-CYS and 229 for PAP-CYS, curtain gas 25 psi, collision gas medium, ion spray voltage 3000 V, temperature ambient, ion source gases 16 psi/0 psi, entrance potential 10 V, declustering potential 60 V, collision cell exit potential 10 V, collision energy 25 V