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For example, neurexin-1 and LRRTM4 bind in a Ca 2+ -dependent manner [61], which may suggest that synaptic adhesion molecules can sense synaptic activity (Ca 2+ release) and thereby cause a change in protein structure to form a protein conformation for binding by both pre- and postsynaptic adhesion molecules, leading to the formation of transsynaptic adhesion molecules

And it is definitely not a reason to abandon a protocol that may be working

Radoslav Bozic at the University of Zagreb, Croatia

Our findings provide novel insights into the microvascular consequences of age-related IGF- 1 deficiency and highlight endothelial IGF- 1R as a potential therapeutic target for preventing vascular dysfunction and sarcopenia in aging muscle