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The statistical analysis of the immunofluorescence images, Massons trichrome staining, and immunohistochemistry results were based on three independent repetitions

Unlike single-pathway therapies, Tirzepatide is a dual-action medication that activates both GLP-1 and GIP receptors simultaneously addressing appetite, satiety, insulin response, and fat metabolism

As a GLP-1 receptor agonist, semaglutide mimics the actions of endogenous GLP-1 but with one critical engineering advantage: while native GLP-1 has a biological half-life of only a few minutes before being cleaved by the enzyme DPP-4, semaglutide achieves a half-life of approximately seven days through structural modifications that include an Aib amino acid substitution at position 8 (blocking DPP-4 recognition) and a C18 fatty acid chain attached via a linker, which enables reversible binding to albumin in the bloodstream

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