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Acoustic calibration was performed with TDT software (SigCal) and thresholds were expressed as dB SPL in conditions identical to that of threshold recordings in animals

These include reduced microbial diversity, decreased abundance of beneficial taxa such as Bifidobacterium and Faecalibacterium prausnitzii , and increased representation of pro-inflammatory microbes (Khavandegar et al., 2024

Blood was collected in EDTA tubes and centrifuged at 1,600 g for 16 min at room temperature to separate the plasma, which was aspirated and stored at 80 C

DSIP dosage protocols can help promote deep, restorative delta-wave sleep and support healthy stress response