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While microneedle technology effectively overcomes the stratum corneum barrier, its clinical application remains constrained by low drug-loading capacity and insufficient mechanical strength (Han et al., 2025)

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Specifically, 1.25 g of dried MSU powder was weighed and placed in a centrifuge tube, to which 36 mL 0.9% sterile sodium chloride solution and 4 mL of Tween 80 were added, resulting in a 25 mg/mL MSU suspension