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Additionally studies on zinc supplementation found reductions in body weight, but only when combined with a calorie-restricted diet

Identification of antibiotic resistance genes was done by doing a sequence homology search (BLASTn) using the online tools of the specific database

The FDA has acknowledged the reports but has not issued an official warning about Ozempic despite the growing number of patients complaining of gastroparesis and eye conditions

The supernatant of protein extracts was heated at 70 C for 10 min in SDS loading buffer (2% (w/v) SDS, 10% (w/v) glycerol, 0.01% (w/v) bromophenol blue and 0.125 M Tris-HCl, 0.3 M DTT, pH 6.8) and loaded on SDS-PAGE acrylamide gels for western blotting