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Ten milliliter of blood was transferred into tubes containing the anticoagulant EDTA to separate red blood cells

After exposure to immunostimulants, there is an enhancement of cytokines in the fetal brain and maternal serum, amniotic fluid and placenta (Urakubo et al., 2001)

(86) conducted a randomized, double-blind, placebo-controlled trial over 8 weeks with 40 patients prone to episodic migraines, finding that omega-3 PUFAs supplementation increased anti-inflammatory cytokine IL-4 and decreased pro-inflammatory cytokine IFN- levels, suggesting a potential beneficial effect on inflammatory responses in migraine patients