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5.2 NRF2-mediated regulation of the pentose phosphate pathway Mechanistically, under conditions of inflammation and oxidative stress, NRF2-mediated activation of the PPP promotes NADPH production, thereby providing reducing equivalents necessary for glutathione regeneration and reactive oxygen species scavenging

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Materials and methods Materials Anhydrous copper chloride (CuCl 2 ), 3,3,5,5-tetramethylbenzidine (TMB), bovine serum albumin (BSA), and sodium sulfide nonahydrate (Na 2 S9H 2 O) were from Macklin Biochemical (Shanghai, China)