Deficiency has been recognized in infants of vegetarian mothers who were breast fed, even though the mothers had no symptoms of deficiency at the time
The mechanism is well mapped
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In the foundational 2017 Cell study, naturally aged mice and chemotherapy-treated mice received FOXO4-DRI at 5 mg/kg by intraperitoneal (IP) injection , given on an intermittent schedule (about three times per week) across several weeks