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The truncated side products are often structurally similar to the full peptide and can share chemical properties, making their separation difficult during stage 1 bulk removal and requiring high-resolution purification

With a vitamin B12 deficiency comes the risk of suffering from megaloblastic anemia, including symptoms of fatigue, weakness, pale skin, lack of appetite, infertility, and unexplained weight loss

doi: 10.1038/s41598-022-15929-6 88 AshrafiNShareghiBFarhadianSHosseini-KoupaeiM

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