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The anti-AKT (1:1000, ab227385), anti-phospho-AKT1 (Ser 473) (1:1000, ab81283), anti-FOXO3a (1:1000, ab12162), and anti-phospho-FOXO3a (Ser 253) (1:1000, ab154768) antibodies were purchased from Abcam Corp

176 In particular, C18- and C16-long-chain ceramides that are produced by ceramide synthase isoforms (CerS1, CerS5, and CerS6) have higher concentrations in insulin-resistant tissues

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