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Animals were then given an intracranial injection of 40 nmol TAT or TAT-DAT NT , and were immediately returned to open-field chambers for a 60-min recording session

This approach is particularly beneficial in trials involving multiple treatment arms or stratified subgroups, where conventional fixed designs often prove too rigid or inefficient

The injection, which doctors deliver into the muscle, typically contains vitamin B12 as cyanocobalamin or hydroxocobalamin