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To validate the candidate genes from CRISPR-Cas9 knockout screening, the top two sgRNAs for each candidate gene, one positive control (sgRNA targeting GGCX), and one negative control (non-targeting sgRNA) were individually cloned into the lentivirial plasmid backbone lentiCRISPR v2 which contains the Cas9 component

Recommended Daily Allowance (RDI) and Population Requirements Intake(PRI) Nutritional requirements depend on the specific needs of individuals , especially for iron, which in turn depend on sex, age and physiological status (pregnancy, breastfeeding women), etc

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Kissen, R., Overby, A., Winge, P