glutathione mitochondrial function Depletion in Diseases Glutathione (GSH) and antioxidant enzymes,

Urbani I, Garvas M, Kokot B, Majaron H, Umek P, Cassidy H, karabot M, Schneider F, Galiani S, Arsov Z, Koklic T, Matallanas D, eh M, Muevi I, Eggeling C, trancar J

Beads were washed with low salt buffer (0.1% SDS, 1% Triton X100, 2 mM EDTA, 20 mM TrisHCl, pH 8, and 150 mM NaCl), high salt buffer (0.1% SDS, 1% Triton X100, 2 mM EDTA, 20 mM TrisHCl, pH 8, and 500 mM NaCl), LiCl buffer (250 mM LiCl, 1% NP40, 1% NaDOC, 1 mM EDTA, and 10 mM TrisHCl, pH 8), twice with TE buffer(10 mM TrisHCl, pH 8, and 1 mM EDTA), and finally with elution buffer (1% SDS and 100 mM NaHCO 3 )

Time course of oxidant markers and antioxidant defenses in subgroups of amyotrophic lateral sclerosis patients

J Clin Endocrinol Metab (2018) 103:366877