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Each ganglion was cut into five pieces, placed in HibernateA minus Ca 2+ media (BrainBits) containing 1 mg/ml collagenase/dispase (Roche Diagnostics) and incubated for 90 min at 37 C

Fibrosis remains the biggest challengeImprovements in fibrosis appear secondary to reducing metabolic and inflammatory injury - Combination approaches, including anti-fibrotic therapies, will likely be needed What This Means for Clinicians: - We are moving toward combination, mechanism-based therapy rather than single-drug approaches - Understanding the gutliver and adiposeliver axes is becoming central to treatment decisions - Patient selection will matter

ARNIs have demonstrated cardiometabolic benefits in existing heart failure studies

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