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[DOI] [PMC free article] [PubMed] [Google Scholar] 126.Barducci R.S., Santos A.A.D., Pacheco L.G., Putarov T.C., Koch J.F.A., Callegari M.A., Dias C.P., de Carvalho R.H., da Silva C.A

Then pellets were resuspended in lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail (Thermo Scientific, 1861280), thoroughly vortexed for 1 min and incubated for 15 min on ice

Mice were intraperitoneally injected with 3% thiolglycollate medium, and mouse peritoneal macrophages (MPMs) were collected after 72 h

Using the avocado to test the satiety effects of a fat-fiber combination in place of carbohydrate energy in a breakfast meal in overweight and obese men and women: a randomized clinical trial