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These were allowed to recover at room temperature for at least one hour in a recovery solution containing 2 mM CaCl 2 , 12.5 mM glucose, 2.5 mM KCl, 2 mM MgCl 2 , 119 mM NaCl, 26 mM NaHCO 3 , 1.25 mM NaH 2 PO 4 , 2 mM thiourea (Sigma, St

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