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How peptide immunotherapy achieves tolerance The mechanisms include induction of specific T-cell anergy (functional inactivation of allergen-reactive T-cells), differentiation of naive T-cells into regulatory T-cells, immune deviation (shifting the Th2/Th1 ratio toward Th1), and allergen-specific Th2 cell deletion

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Total RNA was extracted with Trizol reagent, and cDNA was synthesized from RNA using the Color Reverse Transcription Kit (with gDNA Remover) as per the manufacturer's instructions

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