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Data acquisition and image processing The purified tirzepatideGIPRG s Nb35 complex at a concentration of 18-20 mg/mL was mixed with 100 M tirzepatide at 4 C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific)

At the low end (20 units): 0.40 mg per injection per peptide

[DOI] [PubMed] [Google Scholar] 50.Puig S., Mira H., Dorcey E., Sancenon V., Andres-Colas N., Garcia-Molina A., Burkhead J.L., Gogolin K.A., Abdel-Ghany S.E., Thiele D.J., et al