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Additionally, in rat cardiomyocytes, the p38 MAPK/myocyte enhancer factor 2 axis is a strong inducer of GLUT4 expression (171)

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Subsequently, the HUVECs are stained with a cell-permeable dye calcein AM (Corning, catalog number 354216) for 30 min, and then observed for capillary-like tubule formation using an inverted microscope