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The single-cell quantitative RTPCR (qRTPCR) was performed at the Federal Institute of Technology (ETH) Zurich, Department of Biosystems Science and Engineering (D-BSSE) and Genomics Facility in Basel by using C1 Single-Cell Autoprep and BioMark HD instruments (Fludigim, San Francisco, CA)

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Older men with low serum IGF-1 have an increased risk of incident fractures: the MrOS Sweden study