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Lower Inflammation ApoE-null mice (8 weeks) were infused with vehicle or recombinant human IGF-1 and fed a high-fat diet for 12 weeks

In some patients, lifestyle changes such as reducing protein intake and increasing dietary fiber may be necessary

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The quality of sequence reads was confirmed using FastQC (Babraham Bioinformatics) and low-quality reads and bases were trimmed using Trimmomatic v.0.3 ( -3 were considered significant and clustered based on differential expression compared to untreated monocytes ( Supplementary Table 3A )