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Although glutamate is the major downstream product of glutamine, glutamate supplementation during glutamine deprivation cannot rescue the impaired cell growth or mitochondrial respiration 16,43,44,45 , indicating that mitochondrial GLS-catalyzed cleavage of the gamma-nitrogen of glutamine is essential for glutaminolysis

As a stationary phase, an XBridge Amide Column 3.5 m (2.1 100 mm) (Waters, Milford, MA) was used

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