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Key features of the SCIEX 7500 system for the quantification of acylcarnitines A high-throughput LC-MS method able to quantify acylcarnitine species A highly sensitive method that does not require derivatization of samples before analysis A robust method with high sensitivity and reproducibility and broad dynamic range (LDR) Methods Sample preparation: Labeled and unlabeled standards were purchased from Cambridge Isotopes (p/n: NSK-B-1, NSK-B-US-1)

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Panel A shows the 95th percentile (blue), the mean (green), and the 5th percentile (red) of estradiol during the menstrual cycle